| 质粒名称: | G1333 DddAtox-C–dSpCas9 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 157834 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 完整质粒大小: | 8559bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Mach1 |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS |
|
文献引用方法 |
|
| 材料和方法部分: | G1333 DddAtox-C–dSpCas9 was a gift from David Liu (Addgene plasmid # 157834 ; http://n2t.net/addgene:157834 ; RRID:Addgene_157834) |
| 参考文献部分: | A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, Hsu F, Radey MC, Peterson SB, Mootha VK, Mougous JD, Liu DR. Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4. 10.1038/s41586-020-2477-4 PubMed 32641830 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS G1333 DddAtox-C–dSpCas9 8559 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION expresses split DddAtox-Cas9 construct in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS G1333 DddAtox-C–dSpCas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8559)
AUTHORS Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, Hsu F,
Radey MC, Peterson SB, Mootha VK, Mougous JD, Liu DR
TITLE A bacterial cytidine deaminase toxin enables CRISPR-free
mitochondrial base editing.
JOURNAL Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi:
10.1038/s41586-020-2477-4.
PUBMED 32641830
REFERENCE 2 (bases 1 to 8559)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8559
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 132..335
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 286..306
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 377..396
/label=T7
/note="T7 promoter, forward primer"
promoter 377..395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 445..465
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 844..4944
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 4975..5223
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5254..5502
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5545..5565
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5574..5591
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5614..5631)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5620..5844
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(5915..5931)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5915..5931)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5928..5950)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5939..5955
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5963..5993)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6008..6029
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6146..6163)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6317..6905)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6397..6416)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7076..7936)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7699..7718
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7937..8041)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8120..8139)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 8311..131
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcccaac cccgtaccca
481 aactatgcca atgccggtca tgtggagggt cagagcgccc tgttcatgcg tgataacggc
541 atcagcgagg gtctggtgtt ccacaacaac ccggaaggca cctgcggttt ttgcgtgaac
601 atgaccgaga ccctgctgcc ggaaaacgcg aaaatgaccg tggtgccgcc ggaaggtgcc
661 attccagtga agcgcggcgc taccggtgaa accaaagtgt ttaccggtaa cagcaacagc
721 ccgaagagcc cgaccaaagg cggttgctct ggaggatcta gcggaggatc ctctggcagc
781 gagacaccag gaacaagcga gtcagcaaca ccagagagca gtggcggcag cagcggcggc
841 agcgacaaga agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg
901 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
961 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
1021 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
1081 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
1141 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
1201 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
1261 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
1321 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
1381 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
1441 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
1501 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac
1561 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
1621 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
1681 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
1741 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
1801 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
1861 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
1921 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
1981 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
2041 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
2101 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
2161 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
2221 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
2281 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
2341 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
2401 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
2461 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
2521 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
2581 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
2641 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
2701 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
2761 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
2821 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
2881 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
2941 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
3001 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
3061 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
3121 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
3181 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc
3241 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
3301 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggacgct
3361 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc
3421 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
3481 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg
3541 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
3601 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
3661 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc
3721 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
3781 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
3841 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
3901 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
3961 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
4021 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt
4081 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg
4141 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc
4201 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc
4261 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
4321 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
4381 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
4441 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg
4501 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
4561 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
4621 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
4681 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag
4741 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc
4801 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa
4861 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
4921 gacctgtctc agctgggagg tgacagcggc gggagcggcg ggagcggggg gagcactaat
4981 ctgagcgaca tcattgagaa ggagactggg aaacagctgg tcattcagga gtccatcctg
5041 atgctgcctg aggaggtgga ggaagtgatc ggcaacaagc cagagtctga catcctggtg
5101 cacaccgcct acgacgagtc cacagatgag aatgtgatgc tgctgacctc tgacgccccc
5161 gagtataagc cttgggccct ggtcatccag gattctaacg gcgagaataa gatcaagatg
5221 ctgagcggag gatccggagg atctggaggc agcaccaacc tgtctgacat catcgagaag
5281 gagacaggca agcagctggt catccaggag agcatcctga tgctgcccga agaagtcgaa
5341 gaagtgatcg gaaacaagcc tgagagcgat atcctggtcc ataccgccta cgacgagagt
5401 accgacgaaa atgtgatgct gctgacatcc gacgccccag agtataagcc ctgggctctg
5461 gtcatccagg attccaacgg agagaacaaa atcaaaatgc tgtctggcgg ctcaaaaaga
5521 accgccgacg gcagcgaatt cgagcccaag aagaagagga aagtctaacc ggtcatcatc
5581 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
5641 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
5701 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
5761 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
5821 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac
5881 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
5941 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg
6001 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
6061 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
6121 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
6181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
6241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
6301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
6361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
6421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
6481 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
6541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
6601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
6661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
6721 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
6781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
6841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
6901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacac tcagtggaac gaaaactcac
6961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
7021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
7081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
7141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
7201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
7261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
7321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
7381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
7441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
7501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
7561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
7621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
7681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
7741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
7801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
7861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
7921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
7981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
8041 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc
8101 ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
8161 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
8221 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
8281 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
8341 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
8401 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
8461 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
8521 tacggtaaac tgcccacttg gcagtacatc aagtgtatc
//
